Journal: International Journal of Molecular Sciences

Article Title: Human Neurons Form Axon-Mediated Functional Connections with Human Cardiomyocytes in Compartmentalized Microfluidic Chip

doi: 10.3390/ijms23063148

Figure Lengend Snippet: The evaluation of the functional connections between the cocultured hiPSC-neurons and hiPSC-CMs was performed by video microscopy recording of CMs beating during high K + exposure of the neurons. The data are presented as boxplots overlaid by individual datapoints. ( A ) Experimental timeline for the recording of CMs beating during neuronal exposure to a high-K + solution. ( B ) MUSCLEMOTION was used to analyze the videos for the contraction duration (1), time to peak (2), relaxation time (3), and peak-to-peak time (4). ( C ) The response to medium change (MC) was considered the baseline response, to which the immediate (K+) and follow-up (F/U) responses to neuronal high K + exposure were compared. After K + exposure, the contraction duration was observed to decrease, mostly due to a decrease in the time to peak. Furthermore, the peak-to-peak time of cardiomyocyte beating was observed to decrease, indicating an increased beating rate. ( D ) Examples of contraction curves acquired from MUSCLEMOTION analysis of the recorded videos. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The MultiTissue Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) was used to dissociate the EBs, and PSC-Derived Cardiomyocyte Isolation Kit, human (Miltenyi Biotec), was used to isolate CMs as described previously [ ].

Techniques: Functional Assay, Microscopy